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Sanger sequencing is a method of DNA sequencing based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication. Sanger sequencing is the gold standard for sequencing technology in that it provides a high degree of accuracy, long-read capabilities, and the flexibility to support a diverse range of applications in many research areas. Sanger sequencing also supports applications in RNA sequencing and epigenetic analysis.